Molecular Biology Module 3: Transcription
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The locations of splice sites in newly transcribed protein-encoding RNAs are marked for use by
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You have obtained a sample of a small DNA fragment containing a gene. You transcribe mRNA from this DNA and purify it. You then separate the two strands of the DNA and analyze the base composition of each strand and of the mRNA. You obtain the following data: mRNA: 11% A, 37% C, 29% G, 0% T, 22% U Strand 1: 12% A, 38% C, 27% G, 23% T, 0% U Strand 2: 24% A, 27% C, 38% G, 11% T, 0% U Which strand of the DNA is the template strand?
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(USMLoid) The fragment of DNA shown is transcribed. Which of the following is most likely to be a product of that transcription?
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(USMLoid) In eukaryotes, the primary products of transcription of the rRNA genes first move to:
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These are nine sequences from the -35 regions of 9 typical prokaryotic promoters. Which one is most likely to contain the -35 concensus sequence?
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Which one of the following is true of both prokaryotic and eukaryotic transcription?
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You have identified a DNA element (A -top left) that you think affects transcription. To test its character you add A to sequence 5' to a poorly transcribed (+) DNA sequence (B) and then transfer the recombinant back into a test cell type. The locations and orientations of the added sequence and its effect on transcription () are shown in the diagram. A is:
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