שאלה | You create an E.coli bacteria that is diploid for the Lac operon by placing a second copy of the operon on a plasmid. The chromosomal lac operon is I+ P+ O- Z+ Y+ A+. The plasmid copy is I+ P+ O+ Z+ Y+ A+. You grow these bacteria on plates that contain glucose and X-gal (which turns blue when cut by β -galactosidase), and on plates containing lactose and X-gal. The bacteria will:

You create an E.coli bacteria that is diploid for the Lac operon by placing a second copy of the operon on a plasmid. The chromosomal lac operon is I+ P+ O- Z+ Y+ A+. The plasmid copy is I+ P+ O+ Z+ Y+ A+. You grow these bacteria on plates that contain glucose and X-gal (which turns blue when cut by β -galactosidase), and on plates containing lactose and X-gal. The bacteria will:

		Form blue colonies on the lactose plates and die on the glucose plates.
		Die on both plates
		. Form white colonies on the glucose plates and blue colonies on the lactose plates.
		Form blue colonies on both plates.
		Form white colonies on both plates.
		Form white colonies on the lactose plates and die on the glucose plates
		Die on the lactose plates and form white colonies on the glucose plates.

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